Diagnosis of Benzimidazole Resistance in Haemonchus contortus of Sheep by Allele Specific PCR
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منابع مشابه
Diagnosis of Benzimidazole Resistance in Haemonchus contortus of Sheep by Allele Specific PCR
The study was conducted on 162 adult male Haemonchus contortus of sheep collected from Avikanagar, Jaipur and Bikaner regions to diagnose the benzimidazole (BZ) resistance in H. contortus. The BZ resistance is primarily linked with the mutation in β-tubulin isotype 1 gene which substitute phenylalanine (Phe) into tyrosine (Tyr) at the 200 codon of the gene. An allele specific polymerase chain r...
متن کاملGenetics of Resistance to Haemonchus contortus infections in sheep
........................................................................................................................................ 17 Introduction ................................................................................................................................... 17 Materials and Methods ..........................................................................................
متن کاملevaluation of benzimidazole resistance in haemonchus contortus us-ing comparative pcr-rflp methods
background: in order to deworm the ruminants especially of sheep in iran, consumption of benzimidazoles has more than 2 decades history and today farmers are using imidazothiazoles, macrocyclic lactones and mostly benzimidazole compounds (bzs) to treat infected farm animals. it has been demonstrated that the most common molecular mechanism leading to bzsresistance in haemonchus contortus is a ...
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Indirect plate enzyme-linked immunosorbent assay was standardized and evaluated for its effectivenessin immunodiagnosis of haemonchosis in experimental and clinical cases in sheep by using somatic wholeadult antigen of H. contortus. Plate ELISA was standardized using 5 μg/well antigen concentration with1:100 and 1:1000 of sera and conjugate dilution. Indirect plate ELISA was able to demonstrate...
متن کاملImmunological responses of sheep to Haemonchus contortus.
Infections with Haemonchus contortus are a major constraint on ruminant health world-wide. Young lambs are very sensitive to Haemonchus infection. Older lambs and sheep acquire immunity after a continuous or seasonal exposure to the parasite. The mechanisms underlying immunity are still not completely understood. Antibodies, in particular local IgA and IgE, certainly play a role. The role of Ig...
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ژورنال
عنوان ژورنال: Asian-Australasian Journal of Animal Sciences
سال: 2006
ISSN: 1011-2367,1976-5517
DOI: 10.5713/ajas.2007.7